1、Homo sapiens新乡学院生化作业Homo sapiensinsulin, mRNA (cDNA clone MGC:12292 IMAGE:3950204), complete cdsGenBank: BC005255.1GenBankGraphicsgi|13528923|gb|BC005255.1| Homo sapiens insulin, mRNA (cDNA clone MGC:12292 IMAGE:3950204), complete cdsAGCCCTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATGGCCC
2、TGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCCTCTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGGTGGAGCTGGGCGGGGGCCCTGGTGCAGGCAGCCTGCAGCCCTTGGCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCT
3、ACCAGCTGGAGAACTACTGCAACTAGACGCAGCCCGCAGGCAGCCCCCCACCCGCCGCCTCCTGCACCGAGAGAGATGGAATAAAGCCCTTGAACCAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAPrimer pair 1Sequence (5-3) Template strand Length Start Stop Tm GC% Self complementarity Self 3 complementarityForward primer CAGGACAGGCTGCATCAGAA Plus 20 8 27 60.04 55.0
4、0 5.00 1.00Reverse primer GCTGCGTCTAGTTGCAGTAGT Minus 21 399 379 60.40 52.38 8.00 2.00Product length 392Products on potentially unintended templatesNM_000207.2 Homo sapiens insulin (INS), transcript variant 1, mRNAproduct length = 392Forward primer 1 CAGGACAGGCTGCATCAGAA 20Template 8 . 27Reverse pri
5、mer 1 GCTGCGTCTAGTTGCAGTAGT 21Template 399 . 379NM_001185098.1 Homo sapiens insulin (INS), transcript variant 3, mRNAproduct length = 571Forward primer 1 CAGGACAGGCTGCATCAGAA 20Template 8 . 27Reverse primer 1 GCTGCGTCTAGTTGCAGTAGT 21Template 578 . 558NM_001185097.1 Homo sapiens insulin (INS), transc
6、ript variant 2, mRNAproduct length = 418Forward primer 1 CAGGACAGGCTGCATCAGAA 20Template 8 . 27Reverse primer 1 GCTGCGTCTAGTTGCAGTAGT 21Template 425 . 405NM_001243182.1 Homo sapiens ATPase, Cu+ transporting, beta polypeptide (ATP7B), transcript variant 3, mRNAproduct length = 375Forward primer 1 CAG
7、GACAGGCTGCATCAGAA 20Template 4279 .C.A.G.G. 4298Forward primer 1 CAGGACAGGCTGCATCAGAA 20Template 4653 ACA.GT. 4634NM_001005918.2 Homo sapiens ATPase, Cu+ transporting, beta polypeptide (ATP7B), transcript variant 2, mRNAproduct length = 375Forward primer 1 CAGGACAGGCTGCATCAGAA 20Template 3991 .C.A.G
8、.G. 4010Forward primer 1 CAGGACAGGCTGCATCAGAA 20Template 4365 ACA.GT. 4346NM_000053.3 Homo sapiens ATPase, Cu+ transporting, beta polypeptide (ATP7B), transcript variant 1, mRNAproduct length = 375Forward primer 1 CAGGACAGGCTGCATCAGAA 20Template 4612 .C.A.G.G. 4631Forward primer 1 CAGGACAGGCTGCATCAG
9、AA 20Template 4986 ACA.GT. 4967NM_001128849.1 Homo sapiens SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4), transcript variant 1, mRNAproduct length = 2491Forward primer 1 CAGGACAGGCTGCATCAGAA 20Template 270 .ACA.C.T 289Reverse primer 1 GCT
10、GCGTCTAGTTGCAGTAGT 21Template 2760 C.AGC.G. 2740NM_001128848.1 Homo sapiens SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4), transcript variant 7, mRNAproduct length = 2491Forward primer 1 CAGGACAGGCTGCATCAGAA 20Template 196 .ACA.C.T 215Rev
11、erse primer 1 GCTGCGTCTAGTTGCAGTAGT 21Template 2686 C.AGC.G. 2666NM_001128847.1 Homo sapiens SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4), transcript variant 6, mRNAproduct length = 2491Forward primer 1 CAGGACAGGCTGCATCAGAA 20Template 19
12、6 .ACA.C.T 215Reverse primer 1 GCTGCGTCTAGTTGCAGTAGT 21Template 2686 C.AGC.G. 2666NM_001128846.1 Homo sapiens SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4), transcript variant 5, mRNAproduct length = 2491Forward primer 1 CAGGACAGGCTGCATCA
13、GAA 20Template 196 .ACA.C.T 215Reverse primer 1 GCTGCGTCTAGTTGCAGTAGT 21Template 2686 C.AGC.G. 2666NM_001128845.1 Homo sapiens SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4), transcript variant 4, mRNAproduct length = 2491Forward primer 1
14、CAGGACAGGCTGCATCAGAA 20Template 196 .ACA.C.T 215Reverse primer 1 GCTGCGTCTAGTTGCAGTAGT 21Template 2686 C.AGC.G. 2666NM_001128844.1 Homo sapiens SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4), transcript variant 2, mRNAproduct length = 2491
15、Forward primer 1 CAGGACAGGCTGCATCAGAA 20Template 556 .ACA.C.T 575Reverse primer 1 GCTGCGTCTAGTTGCAGTAGT 21Template 3046 C.AGC.G. 3026NM_003072.3 Homo sapiens SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 (SMARCA4), transcript variant 3, mRNAproduct
16、 length = 2491Forward primer 1 CAGGACAGGCTGCATCAGAA 20Template 480 .ACA.C.T 499Reverse primer 1 GCTGCGTCTAGTTGCAGTAGT 21Template 2970 C.AGC.G. 2950NM_001008781.2 Homo sapiens FAT tumor suppressor homolog 3 (Drosophila) (FAT3), mRNAproduct length = 1790Forward primer 1 CAGGACAGGCTGCATCAGAA 20Template
17、 5501 .T.TGCA. 5520Forward primer 1 CAGGACAGGCTGCATCAGAA 20Template 7290 .AG.T.T.G 7271Primer pair 2Sequence (5-3) Template strand Length Start Stop Tm GC% Self complementarity Self 3 complementarityForward primer TCTACCTAGTGTGCGGGGAA Plus 20 175 194 59.96 55.00 4.00 0.00Reverse primer TGCGTCTAGTTGC
18、AGTAGTTCT Minus 22 397 376 59.44 45.45 6.00 3.00Product length 223Products on potentially unintended templatesNM_000207.2 Homo sapiens insulin (INS), transcript variant 1, mRNAproduct length = 223Forward primer 1 TCTACCTAGTGTGCGGGGAA 20Template 175 . 194Reverse primer 1 TGCGTCTAGTTGCAGTAGTTCT 22Temp
19、late 397 . 376NM_001185098.1 Homo sapiens insulin (INS), transcript variant 3, mRNAproduct length = 223Forward primer 1 TCTACCTAGTGTGCGGGGAA 20Template 354 . 373Reverse primer 1 TGCGTCTAGTTGCAGTAGTTCT 22Template 576 . 555NM_001185097.1 Homo sapiens insulin (INS), transcript variant 2, mRNAproduct le
20、ngth = 223Forward primer 1 TCTACCTAGTGTGCGGGGAA 20Template 201 . 220Reverse primer 1 TGCGTCTAGTTGCAGTAGTTCT 22Template 423 . 402Primer pair 3Sequence (5-3) Template strand Length Start Stop Tm GC% Self complementarity Self 3 complementarityForward primer CTACCTAGTGTGCGGGGAAC Plus 20 176 195 59.82 60
21、.00 4.00 1.00Reverse primer CTGCGTCTAGTTGCAGTAGT Minus 20 398 379 57.36 50.00 8.00 2.00Product length 223Products on potentially unintended templatesNM_000207.2 Homo sapiens insulin (INS), transcript variant 1, mRNAproduct length = 223Forward primer 1 CTACCTAGTGTGCGGGGAAC 20Template 176 . 195Reverse
22、 primer 1 CTGCGTCTAGTTGCAGTAGT 20Template 398 . 379NM_001185098.1 Homo sapiens insulin (INS), transcript variant 3, mRNAproduct length = 223Forward primer 1 CTACCTAGTGTGCGGGGAAC 20Template 355 . 374Reverse primer 1 CTGCGTCTAGTTGCAGTAGT 20Template 577 . 558NM_001185097.1 Homo sapiens insulin (INS), t
23、ranscript variant 2, mRNAproduct length = 223Forward primer 1 CTACCTAGTGTGCGGGGAAC 20Template 202 . 221Reverse primer 1 CTGCGTCTAGTTGCAGTAGT 20Template 424 . 405Primer pair 4Sequence (5-3) Template strand Length Start Stop Tm GC% Self complementarity Self 3 complementarityForward primer GGACAGGCTGCA
24、TCAGAAGA Plus 20 10 29 59.75 55.00 5.00 1.00Reverse primer CTGCGTCTAGTTGCAGTAGTTC Minus 22 398 377 59.08 50.00 8.00 2.00Product length 389Products on potentially unintended templatesNM_000207.2 Homo sapiens insulin (INS), transcript variant 1, mRNAproduct length = 389Forward primer 1 GGACAGGCTGCATCA
25、GAAGA 20Template 10 . 29Reverse primer 1 CTGCGTCTAGTTGCAGTAGTTC 22Template 398 . 377NM_001185098.1 Homo sapiens insulin (INS), transcript variant 3, mRNAproduct length = 568Forward primer 1 GGACAGGCTGCATCAGAAGA 20Template 10 . 29Reverse primer 1 CTGCGTCTAGTTGCAGTAGTTC 22Template 577 . 556NM_00118509
26、7.1 Homo sapiens insulin (INS), transcript variant 2, mRNAproduct length = 415Forward primer 1 GGACAGGCTGCATCAGAAGA 20Template 10 . 29Reverse primer 1 CTGCGTCTAGTTGCAGTAGTTC 22Template 424 . 403NM_020778.4 Homo sapiens alpha-kinase 3 (ALPK3), mRNAproduct length = 3578Forward primer 1 GGACAGGCTGCATCA
27、GAAGA 20Template 4146 .GGC.C.C. 4165Forward primer 1 GGACAGGCTGCATCAGAAGA 20Template 7723 .CA.GGG. 7704NM_182914.2 Homo sapiens spectrin repeat containing, nuclear envelope 2 (SYNE2), transcript variant 5, mRNAproduct length = 3401Forward primer 1 GGACAGGCTGCATCAGAAGA 20Template 6554 .C.CC.GT. 6573F
28、orward primer 1 GGACAGGCTGCATCAGAAGA 20Template 9954 T.TT.A.T. 9935NM_015180.4 Homo sapiens spectrin repeat containing, nuclear envelope 2 (SYNE2), transcript variant 1, mRNAproduct length = 3401Forward primer 1 GGACAGGCTGCATCAGAAGA 20Template 6554 .C.CC.GT. 6573Forward primer 1 GGACAGGCTGCATCAGAAGA
29、 20Template 9954 T.TT.A.T. 9935Primer pair 5Sequence (5-3) Template strand Length Start Stop Tm GC% Self complementarity Self 3 complementarityForward primer ACAGGCTGCATCAGAAGAGG Plus 20 12 31 59.75 55.00 5.00 1.00Reverse primer GCTGCGTCTAGTTGCAGTAGTT Minus 22 399 378 60.93 50.00 8.00 0.00Product length 388Products on potentially unintended t
