1、pColdI pColdII pColdIII pColdTF原核共表达质粒：pACYCduet-1,pETduet-1,pCDFduet-1，pRSFduet-1 Takara公司大肠杆菌分子伴侣：pG-KJE8 pGro7 pKJE7 pGTf2 pTf16大肠杆菌宿主细胞：DH5a JM101 JM103 JM105 JM107 JM109 JM110 Top10 Top10F BL21（DE3） HB101 ER2529 E2566 C2566 MG1655 XL-10gold XL blue M15 JF1125 K802 SG1117 BL21（AI） BL21（DE3）plysS
2、 TG1 TB1 DH5a（pir） Tuner（DE3） Bl21 codonplusRIPL Novablue（DE3） Rosetta Rosetta（DE3） Rosetta（DE3）plys Rosetta-gami（DE3） Rosetta-gamiB（DE3）, Rosetta-gamiB（DE3）plysS Orgami（DE3） OrgamiB（DE3） HMS174（DE3）植物表达/RNAi载体农杆菌pBI121,pBI121-GFP,pBI101,pBI221,pSN1301, pUN1301,pRTL2 , pRTL2-GFP , pRTL2-CFP, pRTL2-R
3、FP , pRTL2-YFP,pCAMBIA 1300, 1301, 1302,1303,1304,1305, 1381Z,1391Z,2300, 2301,3300,3301,pCAMBIA super1300,pCAMBIA super1300-GFP,pPZP212,pPZP2121,pPZP212-GFP,pGDG,RNAi载体pART27,pHANNIBAL,pKANNIBAL, pFGC5941,pTCK303, pTRV1,pTRV2, T-DNA插入载体（随机突变体库）pSKI015,pSKI074,真菌ATMT载体pBIG2RHPH2-GUS-GFP,pBHt1枯草芽孢杆菌表
4、达载体pWB980,pHT43,pHP13,pHP43, pBE2,pMUTIN4,pUB110,pE194,pMA5, pMK3,pMK4,pHT304,pHY300PLK, pBest502,pDG1363,pSG1154,pAX01, pSAS144,pDL,pDG148-stu,pDG641, pAL12,pUCX05-bgaB,pHT01，配套菌株BS 168,WB600,WB800,WB700, WB800N,1012,FZB42,1A747,广宿主质粒pVLT33RNAi基因沉默干扰敲除载体pSilencer1.0，pSilencer 2.1-U6 hygro，pSilencer
5、 3.1-H1 hygro，pSilencer 3.1-H1 neo，pSilencer 4.1-CMV neo，pSilencer 4.1-CMV puro pMIR-REPORT Luciferase RNAi载体（oligoengine） pSuper-puro RNAi逆转录病毒载体（clontech）: RNAi-Ready pSIREN-Retro Q，RNAi-Ready pSIREN-RetroQ-ZsGreen（Luciferase shRNA Annealed Oligonucleotide） RNAi慢病毒载体（addgene）: pLKO.1哺乳动物表达载体pcDNA3
6、.1+/-,pcDNA4/HisMax B，pSecTag2 A，pVAX1，pBudCE4.1,pTracer CMV2，pcDNA3.1（-）/myc-His A，pcDNA6-Myc/His B，pCEP4，pIRES，pIRESneo，pIRES hyg3，pCMV-myc，pCMV-HA，pIRES-puro3，pIRES-neo3,pCAGGS哺乳动物双杂交系统pACT，pBIND，pACT-MyoD，pBIND-Id，pG5luc,pCMV-BD, pCMV-AD, pBD-p53, pFR-luc,Cytotrap Two-Hybrid System:pSos, pSos MA
7、FB, pMyr蜕皮激素诱导系统pIND, pVgRxR,LacSwith II哺乳动物诱导表达系统:pOPRSVI，pOPI3CAT，pCMVLacI,GeneSwitch System:pSwitch哺乳动物表面展示系统:pDisplay,四环素调控系统（Invitrogen）：pcDNA4/TO/Myc-His A，pcDNA4/TO/Myc-His B，pcDNA4/TO/Myc-His C，pcDNA4/TO/Myc-His/LacZ，pcDNA6/TR四环素调控系统（Clontech）：pTet-On，pTet-Off，pTRE2,pRevTRE，pRevTet-On，pRevTe
8、t-off信号通路报告载体:pGAS-TA-Luc，pSTAT3-TA-Luc, pISRE-TA-Luc, pTA-Luc，pIB-EGFP，pNFAT-TA-Luc，pCaspase3-sensor，pAP1（PMA）-Luc;pGL4.26luc2P/minP/Hygro,pGL4.29luc2P/CRE/Hygro,pGL4.30luc2P/NFAT-RE/Hygro，pGL4.75;p53-Luc，pAP-1-Luc，pNF-B-Luc，pSRE-Luc，pFA2-Elk1，pFC-MEKK，pFR-luc,Gateway系统（invitrogen）pcDNA6.2-GWEmGFP-
9、miR negative, pLenti 6/TR,pcDNA 6.2-GW EmGFP-miR，乳酸菌表达载体及各种乳酸菌乳酸杆菌菌株，pNZ8148,pLEISS,pMG36e,pBBR1MCS-5,pBBR1MCS-6,pRV610,pLEM415,pHY300PLK,分泌型乳酸菌表达载体pVE5523，pPG611.1,pPG612.1等和乳酸杆菌菌株宿主菌NZ9000,MG1363,Lactobacillus casei 1.539,Lactobacillus casei,acidophilus NCFM,1.2,Lactobacillus sakei 23K,L.plantarum
10、,L.rhamnosus GG,B.coagulans,Bifidobacterium bifidum,Bifidobacterium infantis,Lactococcus lactis M17,1663,Lactobacillus reuterii广宿主表达载体链球菌表达敲除载体假单胞菌表达载体pVLT33,pBBR1MCS-2,3,4,5,6, pJRD215,pJN105,pME6032,Cos载体pLAFR3,pMP2444（GFP）, pHY300PLK,pRT102,pRL1063a,转座子载体pUT-miniTn5,pMGS100, pWHM10,pKC1139,pSET15
11、2, pOJ260,pPG611.1,pPG612.1，腺病毒载体/慢病毒，逆转录病毒表达载体及包装包膜质粒，腺病毒系统（Stratagene）: pAdEasy-1,pShuttle-CMV,pShuttle,pAdTrack, pAdTrack-CMV, pShuttle-IRES-hrGFP-1、pShuttle-IRES-hrGFP-2、pShuttle-CMV-lacZ, pShuttle-CMV-EGFP-C,pXC1, pBHGE3,配套大肠杆菌BJ5183,293,293T cell line腺相关病毒系统（Stratagene）：pAAV-MCS，pAAV-RC，pHelpe
12、r，pAAV-LacZ，pAAV-IRES-hrGFP，pCMV-MCS，慢病毒载体:pLVX-DsRed-Monomer-N1,pLVX-IRES-ZsGreen1,pLVX-AcGFP1-N1,Lenti6/v5-EDST-EGFP,pWPXL, FUGW,pLentilox 3.7,RNAi-Ready pSIREN-Retro Q, RNAi-Ready pSIREN-Retro Q-ZsGreen, pSUPER.Retro-GFP/Neo,pSUPER-Retro-Neo, pSUPER.Retro-puro,PLNCX PLNCX2 pMSCV-HYG pMSCV-neo pMS
13、CV-puro pLEGFP-C1 pLOX-CW-CRE pLOX-GFP-IRES-TK pRetroX-IRES-DsRedExpress, pLVX-IRES-mCherry质粒载体。病毒包装：二质粒包装系统一：psPAX2 pMD2.G二质粒系统二：pCMV-dR8.2 dvpr pCMV-VSVG三质粒包装系统具有更高的包装效率：pMDLg/pRRE pRSV-Rev pMD2.G Invitrogen包装系统：PLP1 PLP2 PLP/VSVG基因重组载体敲除载体自杀质粒RED重组系统载体pKD3,pKD13,pKD4,pCP20,pKD46, pCVD442,pk18mobs
14、acB,p2NIL, pMAD（革兰氏阳性菌基因敲除），pBT2,RN4220,pKC1139,pOJ260, pJQ200SK,pSET152,pWHM10, pk18mobsacB, pGOAL17,pGOAL19,基因打靶载体PL253，PL451，PL452，PL453,配套菌株EL250,EL350,pMLM290,292, KJBAC1,pST1374荧光蛋白表达载体荧光定位质粒报告基因载体pEGFP,pEYFP,pECFP,pDsRED, pmCherry,pAcGFP,hrGFP,ZsGreen，pDsRed1-C1, pDsRed1-N1, pDsRED-Monomer-N1
15、, pEYFP-C1, pEYFP-N1, pEGFP-1，pEGFP-C1, pEGFP-N1, pEGFP-N3, pcDNA-EGFP1,pECFP-N1,pECFP-C1, pAcGFP1-1 pDsRED2-ER, pDsRED2-Mito, pDsRED2-Nuc，pDsRED2-Peroxi , pEYFP-Golgi, pEYFP-Mem, pEYFP-ER , pEGFP-Actin, pECFP-ER, pIRES2-EGFP, pLEGFP-C1，pLEGFP-N1，pDsRED-Express-N1,pIRES2-DsRed,pIRES-AcGFP1,pmCherry-
16、C1,N1启动子增强子报告载体（Promega，带有Luc荧光报告基因）：pGL3-basic，pGL3-control，pGL3-enhancer，pGL3-promoter，pRL-TK，pRL-CMV，pRL-SV40,信号通路报告载体（Clontech）：pGAS-TA-Luc，pSTAT3-TA-Luc, pISRE-TA-Luc, pTA-Luc，pIB-EGFP，pNFAT-TA-Luc，pCaspase3-sensor，pAP1（PMA）-Luc信号通路报告载体（Promega）：pGL4.26luc2P/minP/Hygro,pGL4.29luc2P/CRE/Hygro,pG
17、L4.30luc2P/NFAT-RE/Hygro，pGL4.75信号通路报告载体（Stratagene）：p53-Luc，pAP-1-Luc，pNF-B-Luc，pSRE-Luc，pFA2-Elk1，pFC-MEKK，pFR-luc昆虫表达载体杆状病毒表达载体系统pFastBac1,pFastBac1-Gus,pFastBacHT A pFastBacHT-CAT,pFastBacDual,pBlueBacHis2 A DH10Bac配套大肠杆菌,pMIB v5-His B ,pIZT/V5-His，Taq,Pfu高表达载体菌株KOD, T4连接酶高表达质粒载体,高产基因工程菌株、酶基因KOD
18、,T4 kinase激酶，Tth反转录酶MMLV，Klentaq PCR酶，Cre酶，RNA inhibitor抑制酶，DL2000质粒BiFC双分子荧光表达载体FRET系统载体pBiFC-VC155，pBiFC-VN173，pBiFC-bFosVC155，pBiFC-bFos（deltaZIP）VC155，pBiFC-bjun-VN173，pCE-BiFC-VN173，pCE-BiFC-VC155，真菌表达载体黑曲霉米曲霉黄曲霉表达质粒pBC-hygro,pAN7-1,pBARGPE1,真菌ATMT农杆菌介导转化载体pBHt1,pBIG2RHPH2-GFP-GUS等，酵母单杂交三杂交载体，酵
19、母双杂交系统pGBKT7，pGADT7，pCL1，pGBKT7-53，pGADT7-T，pGBKT7-Lam，配套酿酒酵母AH109酵母单杂交系统（Clontech）:pHISi,pLacZi,pHis2,pHIS2.1酵母三杂交系统:pBridge, YBZ1菌株Cytotrap Two-Hybrid System（Stratagene）: pSos, pSos MAFB, pMyr噬菌体展示系统载体1.噬菌体展示抗体scFv质粒：pCANTAB5E宿主菌：TG1辅助噬菌体：M13K07可溶表达菌：HB2151 2.噬菌体展示抗体Fabpcomb3XL1-BlueVCSM13pEVOL，pB
20、udCE4.1,pBudCE4.1-EGFP,Zeocin,pEGFP-C3,2,1,pmCherry-C1,-N1,pLOVE-miR302/367,pDsRed2-N1,pAcGFP1.1,TALE toolbox kit,pIRES-hyg3,pIRES-Neo3,pIRES-puro3,pSIREN-RetroQ-ZsGreen,GST 14-3-3,pDsRed-Monomer-N1,M50 super8x TOPFlash,pRK793,pCMV-tag5B,pCMV-tag2B，pCMV-N-HA，pUAST,pw-25,PAC-5.1-HISA，pSLIK-Neo,pCMV-m
21、yc-cyto,pCMV-myc-nuc,疫苗表达载体pVAX1,pAdEasy-1,pShuttle,pAdTrack,pAdTrack-CMV,-polyA,pShuttle-EGFP,BJ5183,pXC1,pBHGE3,pSUPER-retro-GFP-Neo,pORF-lacZ，pORF-TK，pCOX2-Luc,pcDNA3.1（-）-myc-His A,pcDNA3.1-p300,pINP, pOprE, pCR2.1 TOPO,pLP1,pLP2,pLP/VSVG,Stbl3,plenti6/V5-DEST,pLVX-shRNA1,2,pLVX-IRES,tdTomato,FU
22、W-teto-hOCT4,hSOX2,hMYC,hKLF4,FUW-M2rtTA,pSecTag2 A,pEYFP-C1,pEGFP-N1,-C1,pcDNA3.1-V5-His A,B,C,pcDNA3.1-EGFP,pLEGFP-C1,-MHC Promoter,Marc-145猴肾细胞，Sf9，BmN昆虫家蚕细胞，酵母载体pYES2,pYES2.0,INVSc1,pYES3,pYES6,pRS41H,pRS303,304,305，306,pRS424,425,426,414,415,416,pY13,14,15,16,YBZ1,L40酵母菌，pESC-His,pYIP5,pHIS2,pH
23、IS2.1,pHISi,pLacZi,酵母重组载体pUG6,pSH47,植物载体pCAMBIA1300-EGFP, pTCK303,pTRV1,pTRV2,pFGC5941,pUN1301,pSN1301,C58C1，R1601,AGL1农杆菌感受态，原核载体pQE3,pQE30,31,32,pQE60,61,62,M15,DH10Bac,Origami（B）DE3,XL1-Blue,Rosetta gamiB（DE3）pLysS,TKB1,ER2566，DB3.1，JM105,TOP10,DH5a,DH5alpha lambda pir,C43（DE3）菌株感受态，pTrcHis A,B,C
24、,pTWIN1,pTYB1,pTYB11,pACYC184,pMAL-c5x,c2x,p5x,c4x,pBV220,221,222,pCold I,II,III,TF,pET22a,pET29a,pET28a,pET39b,pET41a,pET42a,p15A,pET-sumo,pBADHis A,B,C,pBAD24,34,pETDuet-1,pRSFDuet-1,pCDFDuet-1,pACYCDuet-1,原核荧光pQBI63,pMP2444,链球菌表达、敲除载体pKC1139、pOJ260、pWHM3，pSET152，pVA981，多宿主载体pGOAL19,pBBR1MCS-2,-5,
25、-6,pDSK519,pLA2917，pRK2013，pVLT33,pMAD,RN4220金葡菌株，真菌载体pBARGPE1,pAN7-1,pBHt1,pBIG2RHPH2-GFP-GUS,乳酸菌载体MG1363,pNZ8148,NZ9000,pNZ9530,pMG36e,枯草芽孢杆菌载体pHT43,pWB980,pHT300PLK,pMA5,pUTminiTn5,巨大芽孢杆菌表达载体pHIS1525,WH320菌,谷氨酸棒状杆菌表达载体pEC-XK99E,敲除载体pk18mobsacB,pET载体3a, 3d3c-sumo5b11a A+12a A+15b A+16b A+17b A+19b
26、 A+20b A+21a,21b,21d A+22b A+23a,23b A+23a-tev24a K+25b K+26b K+27b K+28a,28b,28c K+28a-sumo28a-p5329a K+30a ,30c K+31b A+32a A+32a pelB A+35b K+39b K+40b K+41a,41b k+42a,42b K+43b A+43.1a A+44a A+48b K+49b K+50b K+pETduet-1pACYCduet-1pCDFduet-1pRSFduet-1pColAduet-1pET HispET DsbApET GSTpET TrxpQE载体
27、PQE2pQE9pQE30,31,32,pQE30UApQE30LacIqpQE40LpQE60pQE70pQE80L其他原核表达载体PBBR1 MCS Cm+PBBR1 MCS-2 K+PBBR1 MCS-3 Tet+PBBR1 MCS-4 A+PBBR1 MCS-5庆大霉素pMal c2x,p2x,5x,5EpBV220,221,222pTrc99a,pTrcCKS，pTrcHis A,B,CpBAD24,33,43,pBADHis A,B,C,pBADmycHisApEZZ18pThio HisA A+pGEX4T-1,4T-2，4T-3，5X-1,6p-1,2t,3xpColdIpColdIIpColdIIIpColdTFpTwin1pRsetA,B,CPLLP-STQpIN-ompApkk223-3pSE380pJLA502pMBP-C，pMB
