pET-22b大肠杆菌表达载体说明.pdf
- 文档编号:3434439
- 上传时间:2023-05-05
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pET-22b大肠杆菌表达载体说明.pdf
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pET-22b(+)编号编号载体名称载体名称北京华越洋生物VECT5200pET-22b(+)pet22b载体基本信息载体基本信息别名别名:
pET22b,pet22b,pET-22b(+)质粒类型质粒类型:
大肠杆菌蛋白表达表达水平表达水平:
高克隆方法克隆方法:
多克隆位点,限制性内切酶载体大小载体大小:
5500bp5测序引物及序列测序引物及序列:
T7:
5-TAATACGACTCACTATAGGG-33测序引物序列测序引物序列:
T7t:
5-GCTAGTTATTGCTCAGCGG-3载体标签载体标签:
N-pelB;C-His载体抗性载体抗性:
氨苄备注备注:
pET22b载体含有PelB信号肽序列,能够将表达的目的蛋白定位在细胞外周质腔。
稳定性稳定性:
瞬时表达Transient组成型组成型:
组成型Constitutive病毒病毒/非病毒非病毒:
非病毒pet22b载体质粒图谱和多克隆位点信息载体质粒图谱和多克隆位点信息pet22b载体简介载体简介pET-22b(+)载体携带有一个N端的pelB信号肽序列,能够将表达的目的蛋白定位于外周质腔,同时载体含有C端His标签。
载体的单一的多克隆位点见上面的环状质粒图谱。
注意:
载体序列是以pBR322质粒的编码规矩进行编码的,所以T7蛋白表达区在质粒图谱上面是反向的。
T7RNA聚合酶启动的克隆和表达区域在质粒图谱中也被标注了出来。
质粒的F1复制子是被定向的,所以在T7噬菌体聚合酶的作用下,包含有蛋白编码序列的病毒粒子能够产生,并启动蛋白表达,同时蛋白表达将被T7终止子序列的作用下终止蛋白翻译。
pet22b载体序列载体序列ORIGIN1ATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAA61GGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTT121TGTTAGCAGCCGGATCTCAGTGGTGGTGGTGGTGGTGCTCGAGTGCGGCCGCAAGCTTGT181CGACGGAGCTCGAATTCGGATCCGAATTAATTCCGATATCCATGGCCATCGCCGGCTGGG241CAGCGAGGAGCAGCAGACCAGCAGCAGCGGTCGGCAGCAGGTATTTCATATGTATATCTC301CTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCC361TATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATC421GTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACC481GATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATG541GTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTT601GCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCG661CATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTAT721GGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTT781ATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCA841GGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAA901TTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGT961TGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCG1021CGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGC1081CTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTA1141TCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTT1201ATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGG1261TACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGC1321GGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCAC1381TCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTT1441TCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAA1501CGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGC1561GGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTT1621AACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCA1681ACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAG1741AAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATT1801AATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTA1861ATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCA1921ACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTG1981TCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCG2041AGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCT2101TGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGC2161AGGCCATTATCGCCGGCATGGCGGCCCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGA2221GGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCG2281AGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGG2341TCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTA2401TGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTAT2461TAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCG2521CCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTA2581TCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTT2641ACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCA2701GAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAG2761ACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTT2821TCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTC2881TGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGT2941GTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTA3001TGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCAC3061AGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCG3121CTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGG3181TTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAG3241GCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGAC3301GAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGA3361TACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTT3421ACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGC3481TGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCC3541CCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTA3601AGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTAT3661GTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACA3721GTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCT3781TGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATT3841ACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCT3901CAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTC3961ACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAA4021ACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTA4081TTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGC4141TTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGAT4201TTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTA4261TCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTT4321AATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTT4381GGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATG4441TTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCC4501GCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCC4561GTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATG4621CGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGA4681ACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTA4741CCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCT4801TTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAG4861GGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGA4921AGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAAT4981AAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGAAATTGTAAACGTT5041AATATTTTGTTAAAATTCGCGTTAAATTTTTGTTAAATCAGCTCATTTTTTAACCAATAG5101GCCGAAATCGGCAAAATCCCTTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTT5161GTTCCAGTTTGGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGA5221AAAACCGTCTATCAGGGCGATGGCCCACTACGTGAACCATCACCCTAATCAAGTTTTTTG5281GGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCT5341TGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGC5401GCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTT5461AATGCGCCGCTACAGGGCGCGTCCCATTCGCCA/其他其他大肠杆菌大肠杆菌表达载体:
表达载体:
pBV221ptdTomatopET-52b(+)pAmCyanpDsRed-Express2pBV220pCold-GSTpColdS-SUMOpColdTFpColdIVpColdIIIpColdIIpColdIpE-SUMOpCold-ProS2pBAD102/D-TOPOpBAD202/D-TOPOpACYC184pBAD/Thio-TOPOpBad/Myc-HisCpBad/Myc-HisBpBad/Myc-HisApBad/HisCpBad/HisBpBad/HisApBAD-TOPOpET-23b(+)pET-23a(+)pET-23c(+)pET-23(+)pET-12b(+)pET-12c(+)pET-12a(+)pET-11b(+)pET-11a(+)pET-11c(+)pBad24pQE-82LpQE-81LpQE-80LpQE-32pQE-9pQE-16pQE-31pQE-60pQE-70pQE-40pET-51b(+)pET-50b(+)pET-49b(+)pET-48b(+)pET-47b(+)pET-26b(+)pET-32a(+)pET-21b(+)pET-22b(+)pET-14bpET-16bpET-15bpET-19bpET-20b(+)pET-21d(+)pET-21c(+)pET-21b(+)pET-21a(+)pET-24a(+)pET-24d(+)pET-25b(+)pET-27b(+)pET-28a(+)pET-30a(+)pET-42a(+)pET-43.1c(+)pET-43.1b(+)pET-43.1a(+)pET-44a(+)pET-44c(+)pET-46EK/LICpET-37b(+)pTrcHis2CpTrcHis2BpTrcHis2ApET303/CT-HispET302/NT-HispRSET-CFPpRSET-EmGFPpRSET-BFPpGFPuvpET300/NT-DESTpET301/CT-DESTpGEM-TpBad43pGEX-4T-3pGEX-5X-2pBlueScriptSK(+)pG-Tf2pG-KJE8pGro7pET-SUMOpSE380pET-17bpET102/D-TOPOpCDFDuet-1pMAL-p5xpTf16pET-28c(+)pBluescriptIISK(+)pET-30b(+)pSUMOpProEXHTcpProEXHTbpProEXHTapKD3pKD13pKD46pTYB1pTYB2pTWIN2pBluescriptIIKS(-)pTYB12pMAL-p5epACYCDuet-1pEGM-11ZF(+)pEGM-7ZF(+)PinPointXa-3PinPointXa-2PinPointXa-1pSP73pSP64pTWIN1pTYB11pTXB1pET-5b(+)pBad/gIIICpBad/gIIIBpBad/gIIIApET-5a(+)pMal-p4XpMal-p2Gpkk223-3pkk232-8pCYB1pEZZ18pBAD18pMAL-c5xpMal-p2EpMal-p2XpET-44EK/LICpET-43.1EK/LICpET-41EK/LICpMal-c4XpTrcHisBpET-31b(+)pET-3b(+)pET-41a(+)pGEX-3XpGEX-4T-2pETDuet-1pGEX-4T-1pTrc99apET-28b(+)pET-HispALEXa,b,cpACYC177pBR322pKD4pKD20pMXB10pEcoli-6xHN-GFPuvpKJE7pRSETBpGEX-KGpGEX-2TpRSFDuet-1pCOLADuet-1pTrcHisCpTrcHisApET-41b(+)pET-42b(+)pET-3a(+)pGEX-6P-3pGEX-6P-2pGEX-6P-1pGEX-5X-3pGEX-5X-1pGEX-2TKpRSETApMal-c2GpMal-c2EpMal-c2XpRSETCpQE-30pET-45b(+)pET-44b(+)pET-42c(+)pET-41c(+)pET-40b(+)pET-33b(+)pET-39b(+)pET-32EK/LICpET-32Xa/LICpET-32c(+)pET-32b(+)pET-30Xa/LICpET-30EK/LICpET-30c(+)pET-29c(+)pET-29b(+)pET-29a(+)pET-24c(+)pET-24b(+)pET-24(+)pET-23d(+)pET-11d(+)pBad33
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- pET 22 大肠杆菌 表达 载体 说明